Mass spectral analysis and fragment ion structure of fusarochromanone.

Fusarochromanone is a mycotoxin produced by Fusarium equiseti that is implicated in the poultry disease tibial dyschrondroplasia. Electron impact ionization tandem mass spectrometry was used to elucidate probable structures of fragment ions found at m/z 274, 275, 261, 233, 218 and 191 and for devising an analytical rationale for the metabolites of the parent compound. In addition, a sensitive, qualitative liquid chromatographic technique using direct injection continuous-flow fast atom bombardment for the detection of fusarochromanone in corn was devised. Analysis was carried out on a hybrid tandem instrument (VG-7070EQ) using open tubular columns (75 microns i.d.) with direct-flow open-loop injection. The limit of detection of the pure compound was 500 pg in the selected ion monitoring mode. A 50 p.p.b. (500 pg injected) of the pure compound added to ground corn samples was the lowest detectable amount in a biological matrix.