Barbosa Taciana Fernandes Souza, Medeiros Daniele Barbosa de Almeida, Travassos da Rosa Elizabeth Salbe, Casseb Lívia Medeiros Neves, Medeiros Rita, Pereira Armando de Souza, Vallinoto Antonio Carlos Rosário, Vallinoto Marcelo, Begot Alberto Lopes, Lima Reynaldo José da Silva, Vasconcelos Pedro Fernando da Costa, Nunes Márcio RobertoTeixeira
Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Belém, Brazil.
Virology. 2008 Jan 20;370(2):228-36. doi: 10.1016/j.virol.2007.10.005. Epub 2007 Nov 8.
We genetically characterize rabies virus (RABV) strains isolated from human cases, domestic and wild animals during a human outbreak of bat-transmitted rabies in Augusto Correa municipality, Pará state, Brazilian Amazon in 2005. Partial nucleotide sequences of the N gene (491 bp) were obtained for all strains, and phylogenetic analysis grouped these into two major clades (Pará and Central-Southeast) and identified them as bat-related viruses genotype I, Desmodus rotundus antigenic variant 3 (AgV3). A molecular clock was used to estimate the time of emergence for each RABV isolate. The molecular data from this study suggest the association of vampire bats with human and domestic animal cases reported in the outbreak, the circulation of at least two predominant lineages in the Pará state, and also a geographic association to lineages dispersion.
我们对2005年在巴西亚马逊州帕拉州奥古斯托·科雷亚市发生的由蝙蝠传播的狂犬病人间疫情期间从人类病例、家畜和野生动物中分离出的狂犬病病毒（RABV）毒株进行了基因特征分析。获得了所有毒株N基因的部分核苷酸序列（491 bp），系统发育分析将这些毒株分为两个主要分支（帕拉和中东南部），并将它们鉴定为蝙蝠相关病毒基因型I，圆叶吸血蝠抗原变异体3（AgV3）。使用分子钟来估计每个RABV分离株的出现时间。本研究的分子数据表明吸血蝙蝠与疫情中报告的人类和家畜病例有关联，帕拉州至少有两个主要谱系在传播，并且谱系传播存在地理关联。
