Kank蛋白：一个新的含锚蛋白重复结构域的蛋白家族。

Kank proteins: a new family of ankyrin-repeat domain-containing proteins.

作者信息

Zhu Yun, Kakinuma Naoto, Wang Yong, Kiyama Ryoiti

机构信息

Signaling Molecules Research Laboratory, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan.

出版信息

Biochim Biophys Acta. 2008 Feb;1780(2):128-33. doi: 10.1016/j.bbagen.2007.09.017. Epub 2007 Oct 4.

DOI:10.1016/j.bbagen.2007.09.017

PMID:17996375

Abstract

The human Kank gene was found as a candidate tumor suppressor for renal cell carcinoma, and encodes an ankyrin-repeat domain-containing protein, Kank. Here, we report a new family of proteins consisting of three Kank (Kank1)-associated members, Kank2, Kank3 and Kank4, which were found by domain and phylogenetic analyses. Besides the conserved ankyrin-repeat and coiled-coil domains, there was a conserved motif at the N-terminal (KN motif) containing potential motifs for nuclear localization and export signals. Gene expression of these genes was examined by RT-PCR at the mRNA level and by Western blotting and immunostaining at the protein level. Kank family genes showed variations in the expression level among tissues and kidney cell lines. Furthermore, the results of overexpression of these genes in NIH3T3 cells suggest that all of these family proteins have an identical role in the formation of actin stress fibers.

摘要

人类Kank基因被发现是肾细胞癌的候选肿瘤抑制基因，它编码一种含锚蛋白重复结构域的蛋白质Kank。在此，我们报告了一个由三个与Kank（Kank1）相关的成员Kank2、Kank3和Kank4组成的新蛋白质家族，这些成员是通过结构域和系统发育分析发现的。除了保守的锚蛋白重复结构域和卷曲螺旋结构域外，在N端还有一个保守基序（KN基序），其中包含潜在的核定位和输出信号基序。通过RT-PCR在mRNA水平以及通过蛋白质印迹和免疫染色在蛋白质水平检测这些基因的表达。Kank家族基因在组织和肾细胞系中的表达水平存在差异。此外，这些基因在NIH3T3细胞中过表达的结果表明，所有这些家族蛋白在肌动蛋白应力纤维的形成中具有相同的作用。