Jaleel C Abdul, Sankar B, Murali P V, Gomathinayagam M, Lakshmanan G M A, Panneerselvam R
Stress Physiology Lab, Department of Botany, Annamalai University, Annamalainagar, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2008 Mar 15;62(1):105-11. doi: 10.1016/j.colsurfb.2007.09.026. Epub 2007 Sep 29.
In the present work, we have analysed the changes in the reactive oxygen metabolism of Catharanthus roseus (L.) G. Don. plants in terms of H(2)O(2) content, lipid peroxidation and the free radical quenching systems (non-enzymatic and enzymatic antioxidants) under drought stress. In addition to this, the root alkaloid ajmalicine was extracted and quantified from both control and drought stressed plants. The H(2)O(2) content was analysed from both stressed and unstressed control plants. Lipid peroxidation was estimated as thiobarbituric acid reactive substances. The non-enzymatic antioxidants viz., ascorbic acid, alpha-tocopherol and reduced glutathione contents, antioxidant enzymes like superoxide dismutase, ascorbate peroxidase, and catalase were extracted and estimated from the samples. The alkaloid ajmalicine was extracted and quantified from shade dried root samples and found significantly increased over control. From the results of this investigation, it can be concluded that the water deficit areas may be well used for the cultivation of medicinal plants like C. roseus and the economically important alkaloid production can be enhanced in the plant level.
在本研究中，我们从过氧化氢含量、脂质过氧化以及干旱胁迫下的自由基淬灭系统（非酶和酶抗氧化剂）方面，分析了长春花（Catharanthus roseus (L.) G. Don.）植株活性氧代谢的变化。除此之外，还从对照植株和干旱胁迫植株中提取并定量了根部生物碱阿吗碱。分析了胁迫植株和未胁迫对照植株中的过氧化氢含量。脂质过氧化以硫代巴比妥酸反应性物质来估算。从样品中提取并测定了非酶抗氧化剂，即抗坏血酸、α-生育酚和还原型谷胱甘肽的含量，以及超氧化物歧化酶、抗坏血酸过氧化物酶和过氧化氢酶等抗氧化酶。从阴干的根样品中提取并定量了生物碱阿吗碱，发现其含量比对照显著增加。从本研究结果可以得出结论，缺水地区可很好地用于种植长春花等药用植物，并且可以在植株水平上提高具有经济重要性的生物碱产量。
