Jaleel C Abdul, Gopi R, Manivannan P, Sankar B, Kishorekumar A, Panneerselvam R
Stress Physiology Lab, Department of Botany, Annamalai University, Annamalainagar 608002, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2007 Nov 15;60(2):195-200. doi: 10.1016/j.colsurfb.2007.06.009. Epub 2007 Jun 14.
Changes in antioxidant potentials and indole alkaloid, ajmalicine, production were studied in Catharanthus roseus (L.) G. Don. plants under treatment with gibberellic acid (GA(3)). The GA(3) treatments were given in two ways, foliar spray and soil drenching methods on 30, 45, 60 and 75 days after planting (DAP). The plants were uprooted randomly on 90 DAP and separated into root, stem and leaves and used for analyses. The antioxidant potential was studied in terms of non-enzymatc antioxidant molecules like ascorbic acid (AA), alpha-tocopherol (alpha-toc) and reduced glutathione (GSH) and activities of antioxidant enzyme, viz., superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT). The alkaloid ajmalicine was extracted and estimated from roots of both control and treated plants. It was found that, GA(3) has a profound effect upon the antioxidant potentials and it caused a significant enhancement in the production of ajmalicine when compared to untreated control as well as foliar-sprayed plants. There was no significant enhancement in GSH and ajmalicine content under GA(3) foliar spray in C. roseus. These preliminary results suggest that, the application of GA(3) may be a useful tool to increase the antioxidant potential and alkaloid production in medicinal plants like C. roseus.
研究了赤霉素（GA₃）处理下长春花（Catharanthus roseus (L.) G. Don.）植株抗氧化能力及吲哚生物碱阿吗碱产量的变化。GA₃处理采用两种方式，即种植后30、45、60和75天进行叶面喷施和灌根处理。在种植90天后随机拔起植株，分为根、茎和叶用于分析。从非酶抗氧化分子如抗坏血酸（AA）、α-生育酚（α-toc）和还原型谷胱甘肽（GSH）以及抗氧化酶超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）的活性方面研究抗氧化能力。从对照植株和处理植株的根中提取并测定生物碱阿吗碱。结果发现，GA₃对抗氧化能力有深远影响，与未处理对照以及叶面喷施植株相比，它能显著提高阿吗碱的产量。在GA₃叶面喷施处理下，长春花的GSH和阿吗碱含量没有显著提高。这些初步结果表明，GA₃的应用可能是提高长春花等药用植物抗氧化能力和生物碱产量的有用工具。
