Lynn D E, Hung A C
Insect Biocontrol Laboratory and Bee Research Laboratory, Beltsville Agricultural Research Center, Maryland 20705-2350.
Arch Insect Biochem Physiol. 1991;18(2):99-104. doi: 10.1002/arch.940180204.
Although numerous insect cell lines have been developed over the past three decades, few of these have been from the order Hymenoptera. This report describes two new continuous cell lines from trichogrammid wasps. The extremely small size of these insects has made physiological and biochemical studies difficult. Now, with the development of the cell lines, a limitless supply of biologically active material is available for a wide variety of basic biological studies. The Trichogramma confusum and T. exiguum cell lines (designated IPLB-Tcon1 and IPLB-Tex2) were characterized by chromosome and isozymes techniques. Evidence of their utility is shown by morphological response to the developmental hormone, 20-hydroxyecdysone. The morphological change in IPLB-Tex2 is accompanied by an induction of highly contractile cells which indicates this cell line may be composed of myoblast cells.
尽管在过去三十年中已经建立了许多昆虫细胞系，但其中很少来自膜翅目。本报告描述了两种来自赤眼蜂的新的连续细胞系。这些昆虫极小的体型使得生理和生化研究变得困难。现在，随着细胞系的发展，可获得无限量的生物活性物质用于各种基础生物学研究。用染色体和同工酶技术对混淆赤眼蜂和微小赤眼蜂细胞系（分别命名为IPLB-Tcon1和IPLB-Tex2）进行了鉴定。它们对发育激素20-羟基蜕皮酮的形态学反应证明了其效用。IPLB-Tex2中的形态变化伴随着高收缩性细胞的诱导，这表明该细胞系可能由成肌细胞组成。
