Rondeau-Mouro C, Defer D, Leboeuf E, Lahaye M
UR 1268 Biopolymères Interactions Assemblages, INRA, BP 71627, 44316 Nantes cedex 03, France.
Int J Biol Macromol. 2008 Mar 1;42(2):83-92. doi: 10.1016/j.ijbiomac.2007.09.020. Epub 2007 Oct 6.
The impact of homogalacturonans deficiency on the cell wall porosity of Arabidopsis thaliana QUA1 mutant was investigated using NMR measurements of protons mobility interpreted in terms of pore size variations at nanometer and micrometer scales. Isolation and purification of wild type and mutant stems and calli cell walls permitted to exacerbate the putative impact of the mutation on cellulose-hemicelluloses assembly in highly and poorly organised cell walls, respectively. NMR relaxation measurements of water and exchangeable biopolymer protons and self-diffusion processes of polyethylene glycol in walls informed about the porosity network density and heterogeneity. The role of pectins and proteins as well as the growth status of the cells on the wall porosity regulation are discussed.
利用核磁共振测量质子迁移率来研究同型半乳糖醛酸缺乏对拟南芥QUA1突变体细胞壁孔隙率的影响，该测量结果根据纳米和微米尺度上的孔径变化进行解释。野生型和突变体茎及愈伤组织细胞壁的分离和纯化，分别有助于加剧该突变对高度有序和组织不良的细胞壁中纤维素-半纤维素组装的假定影响。对细胞壁中水和可交换生物聚合物质子的核磁共振弛豫测量以及聚乙二醇在细胞壁中的自扩散过程，提供了有关孔隙率网络密度和异质性的信息。本文还讨论了果胶和蛋白质的作用以及细胞生长状态对细胞壁孔隙率调节的影响。
