Ogura Manabu, Furukawa Masayuki, Tada Naoki, Ikeda Hiroki, Yamashita Toshio
Department of Otolaryngology, Kansai Medical University, 2-3-1 Shinmachi, Hirakata, Osaka, Japan.
Auris Nasus Larynx. 2008 Sep;35(3):338-43. doi: 10.1016/j.anl.2007.09.009. Epub 2007 Nov 13.
We examined the effect of platelet-activating factor (PAF) on IL-8 production in cultured rat middle ear epithelial cells (RMECs), and the concentrations of cytokine, PAF, and PAF-acetylhydrolase (PAF-AH) were also examined in the PAF-induced experimental OME (otitis media with effusion) of rats.
Using an enzyme-linked immunospecific assay, we measured the levels of cytokines in the cultured RMECs and the PAF-induced OME of rats. The PAF was quantitated by the platelet-aggregating activity and the PAF-AH was measured by radioimmunoassay.
Both PAF and C-PAF, which is a stable analogue of PAF, significantly induced production of IL-8 in the RMECs in a dose-dependent manner. The PAF-induced IL-8 production was abolished by co-incubation with WEB2170, a specific PAF receptor antagonist. The concentrations of the cytokines and PAF in the PAF-induced OME of rats were higher on day 1 and the PAF and cytokine levels seemed to correspond well with the persistence of OME.
PAF may stimulate the local production of cytokines and may induce OME in the middle ear.
我们研究了血小板活化因子（PAF）对培养的大鼠中耳上皮细胞（RMECs）中白细胞介素8（IL-8）产生的影响，并且还检测了PAF诱导的大鼠实验性渗出性中耳炎（OME）中细胞因子、PAF和PAF-乙酰水解酶（PAF-AH）的浓度。
我们使用酶联免疫特异性测定法，测量培养的RMECs和PAF诱导的大鼠OME中细胞因子的水平。通过血小板聚集活性对PAF进行定量，通过放射免疫测定法测量PAF-AH。
PAF和C-PAF（PAF的一种稳定类似物）均以剂量依赖性方式显著诱导RMECs中IL-8的产生。与特异性PAF受体拮抗剂WEB2170共同孵育可消除PAF诱导的IL-8产生。在PAF诱导的大鼠OME中，细胞因子和PAF的浓度在第1天较高，并且PAF和细胞因子水平似乎与OME的持续时间密切相关。
PAF可能刺激细胞因子的局部产生，并可能诱导中耳发生OME。
Zotero 插件