Bell Eric L, Chandel Navdeep S
Department of Medicine, Northwestern University Medical School, Chicago, Illinois, USA.
Methods Enzymol. 2007;435:447-61. doi: 10.1016/S0076-6879(07)35023-4.
Oxygen is the terminal electron acceptor in the mitochondrial electron transport chain and therefore is required for the generation of energy through oxidative phosphorylation. In environments of decreased oxygen levels (hypoxia), organisms have developed an adaptive response through the activation of the hypoxia-inducible transcription factor (HIF) to maintain their energetic demand. In order to sense hypoxic environments, cells have developed oxygen-sensing machinery that allows for the activation of HIF. The mitochondrial electron transport chain is required for the oxygen-sensing pathway. This chapter outlines methods used to explore the role of the electron transport chain and a by-product of electron transport, reactive oxygen species, in oxygen sensing.
氧气是线粒体电子传递链中的终端电子受体，因此通过氧化磷酸化产生能量需要氧气。在氧气水平降低的环境（缺氧）中，生物体通过激活缺氧诱导转录因子（HIF）来发展适应性反应，以维持其能量需求。为了感知缺氧环境，细胞已经发展出允许激活HIF的氧气感应机制。氧气感应途径需要线粒体电子传递链。本章概述了用于探索电子传递链的作用以及电子传递的副产物活性氧在氧气感应中的作用的方法。
