Li Ke, Liu Liqiang, Xu ChuanLai, Chu XiaoGang
WuXi KangBaiEr Biotechnology Corporation, WuXi, JiangSu, P.R. China.
Anal Sci. 2007 Nov;23(11):1281-4. doi: 10.2116/analsci.23.1281.
An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g(-1) for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R(2) = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site.
开发了一种免疫层析测定法，用于检测水产养殖组织中的氯霉素（CAP）残留。对于添加到水产养殖组织中的CAP，检测限（LOD）为10 ng g(-1)。结果通过液相色谱串联质谱法（LC/MS/MS）得到证实，表明两种方法之间具有良好的一致性。线性回归方程为y = 1.19x + 0.539，R(2) = 0.978。检测的分析时间少于5分钟，该方法适用于现场快速检测。
