基于侧流核酸生物传感器的 miRNA 可视化检测。
Visual detection of microRNA with lateral flow nucleic acid biosensor.
机构信息
Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, PR China; Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA.
Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, USA.
出版信息
Biosens Bioelectron. 2014 Apr 15;54:578-84. doi: 10.1016/j.bios.2013.10.055. Epub 2013 Nov 25.
We report a DNA-gold nanoparticle (DNA-GNP) based lateral flow nucleic acid biosensor for visual detection of microRNA (miRNA)-215 in aqueous solutions and biological samples with low-cost and short analysis time. Sandwich-type hybridization reactions among GNP-labeled DNA probe, miRNA-215 and biotin-modified DNA probes were performed on the lateral flow device. The accumulation of GNPs on the test zone of the biosensor enables the visual detection of miRNA-215. After systematic optimization, the biosensor was able to detect a minimum concentration of 60 pM miRNA-215. The biosensor was applied to detect miRNA-215 from A549 cell lysate directly without complex sample treatment, and the detection limit of 0.148 million cells was obtained. This study provides a simple, rapid, specific and low-cost approach for miRNA detection in aqueous solutions and biological samples, showing great promise for clinical application and biomedical diagnosis in some malignant diseases.
我们报告了一种基于 DNA-金纳米粒子（DNA-GNP）的侧向流动核酸生物传感器，用于在水溶液和生物样品中可视化检测 microRNA（miRNA）-215，具有低成本和短分析时间的特点。在侧向流动装置上进行了 GNP 标记的 DNA 探针、miRNA-215 和生物素修饰的 DNA 探针之间的三明治型杂交反应。生物传感器上测试区的 GNPs 积累使 miRNA-215 的可视化检测成为可能。经过系统优化，该生物传感器能够检测到最小浓度为 60 pM 的 miRNA-215。该生物传感器可直接用于检测 A549 细胞裂解液中的 miRNA-215，无需复杂的样品处理，检测限为 0.148 万个细胞。这项研究为水溶液和生物样品中的 miRNA 检测提供了一种简单、快速、特异和低成本的方法，有望在某些恶性疾病的临床应用和生物医学诊断中得到应用。
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