Krampera Mauro, Sartoris Silvia, Liotta Francesco, Pasini Annalisa, Angeli Roberta, Cosmi Lorenzo, Andreini Angelo, Mosna Federico, Bonetti Bruno, Rebellato Elisabetta, Testi Maria Grazia, Frosali Francesca, Pizzolo Giovanni, Tridente Giuseppe, Maggi Enrico, Romagnani Sergio, Annunziato Francesco
Department of Clinical and Experimental Medicine, Section of Haematology, University of Verona, Italy.
Stem Cells Dev. 2007 Oct;16(5):797-810. doi: 10.1089/scd.2007.0024.
We show here that human and mouse mesenchymal stem cells (MSCs) can be obtained not only from bone marrow (BM), but also from adult spleen and thymus. In vitro, both human and mouse spleen- and thymus-derived MSCs exhibit immunophenotypic characteristics and differentiation potential completely comparable to BM-MSCs. In addition, they can inhibit immune responses mediated by activated T lymphocytes with efficiency comparable to BM-MSCs. In vivo, mouse MSCs from BM, spleen, and thymus, if injected together with a genetically modified tumor cell vaccine, can equally prevent the onset of an anti-tumor memory immune response, thus leading to tumor growth in normally resistant mice. Our data suggest that not only do spleen and thymus have a stem cell reservoir to build up their stromal architecture, but also contain microenviromental immunoregulatory cells with the same properties of BM-MSCs.
我们在此表明，人和小鼠间充质干细胞（MSCs）不仅可以从骨髓（BM）中获得，还可以从成年脾脏和胸腺中获得。在体外，人和小鼠脾脏及胸腺来源的MSCs均表现出与骨髓间充质干细胞（BM-MSCs）完全可比的免疫表型特征和分化潜能。此外，它们能够以与BM-MSCs相当的效率抑制活化T淋巴细胞介导的免疫反应。在体内，如果将来自骨髓、脾脏和胸腺的小鼠MSCs与基因改造的肿瘤细胞疫苗一起注射，同样可以预防抗肿瘤记忆免疫反应的发生，从而导致正常情况下具有抵抗力的小鼠体内肿瘤生长。我们的数据表明，脾脏和胸腺不仅拥有构建其基质结构的干细胞库，还含有具有与BM-MSCs相同特性的微环境免疫调节细胞。
