控制阴离子脂质双层的电荷与组织：单价和二价离子的作用

Controlling the charge and organization of anionic lipid bilayers: effect of monovalent and divalent ions.

作者信息

Lamberson Emily R, Cambrea Lee R, Hovis Jennifer S

机构信息

Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.

出版信息

J Phys Chem B. 2007 Dec 13;111(49):13664-7. doi: 10.1021/jp076306a. Epub 2007 Nov 15.

DOI:10.1021/jp076306a

PMID:18001085

Abstract

It is shown that the organization of lipid bilayers containing phosphatidic acid (PA) and phosphatidlycholine (PC) can be controlled by altering the monovalent and divalent ion concentrations. At high pH and/or calcium concentration, 1:1 Ca(2+)-PA(2-) complexes form; these complexes demix, and PA-rich and PC-rich regions are observable with epifluorescence microscopy. The results are compared with predictions from electrostatic theory. It is noted that the complex formation correlates in a roughly linear fashion with the monovalent/divalent ion ratio, a parameter that cells adjust.

摘要

结果表明，通过改变单价和二价离子浓度，可以控制含有磷脂酸（PA）和磷脂酰胆碱（PC）的脂质双层的组织结构。在高pH值和/或钙浓度下，会形成1:1的Ca(2+)-PA(2-)复合物；这些复合物会发生相分离，通过落射荧光显微镜可以观察到富含PA和富含PC的区域。将这些结果与静电理论的预测进行了比较。值得注意的是，复合物的形成与单价/二价离子比率大致呈线性相关，而细胞可以调节这一参数。