Imfeld K, Garenne A, Neukom S, Maccione A, Martinoia S, Koudelka-Hep M, Berdondini L
Electronics and Signal Processing Laboratory, Institute of Microtechnology University of Neuchâtel, Rue A.-L. Breguet 2, CH-2000 Neuchâtel, Switzerland.
Annu Int Conf IEEE Eng Med Biol Soc. 2007;2007:6086-9. doi: 10.1109/IEMBS.2007.4353737.
A platform based on an active-pixel-sensor electrode array (APS-MEA) for high-resolution imaging of in-vitro electrogenic cell cultures is presented, characterized and validated under culture conditions. The system enables full frame acquisition at 8 kHz from 4096 microelectrodes integrated with separations of 21 microm and zoomed area acquisition with temporal resolutions down to 8 micros. This bi-modal acquisition feature opens new perspectives in particular for neuronal activity analysis and for the correlation of micro-scale and macro-scale behaviors. The low-noise performances of the integrated amplifier (11 microVRMS) combined with a hardware implementation reflecting image-/video-concepts enable high-resolution acquisitions with real-time processing capabilities adapted to the handling of the large amount of acquired data.
本文介绍了一种基于有源像素传感器电极阵列（APS-MEA）的平台，用于体外电生细胞培养的高分辨率成像，并在培养条件下对其进行了表征和验证。该系统能够以8 kHz的频率从集成有21微米间距的4096个微电极进行全帧采集，并能以低至8微秒的时间分辨率进行缩放区域采集。这种双峰采集功能尤其为神经元活动分析以及微观和宏观行为的相关性开辟了新的视角。集成放大器的低噪声性能（11微伏均方根值）与反映图像/视频概念的硬件实现相结合，实现了具有实时处理能力的高分辨率采集，以适应处理大量采集数据的需求。
