Kanwar R K, Macgibbon A K, Black P N, Kanwar J R, Rowan A, Vale M, Krissansen G W
LactoPharma Consortium, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand.
Clin Exp Allergy. 2008 Jan;38(1):208-18. doi: 10.1111/j.1365-2222.2007.02868.x. Epub 2007 Nov 13.
It has been argued that a reduction in the Western diet of anti-inflammatory unsaturated lipids, such as n-3 polyunsaturated fatty acids, has contributed to the increase in the frequency and severity of allergic diseases.
We investigated whether feeding milk fat enriched in conjugated linoleic acid and vaccenic acids (VAs) ('enriched' milk fat), produced by supplementing the diet of pasture-fed cows with fish and sunflower oil, will prevent development of allergic airway responses.
C57BL/6 mice were fed a control diet containing soybean oil and diets supplemented with milk lipids. They were sensitized by intraperitoneal injection of ovalbumin (OVA) on days 14 and 28, and challenged intranasally with OVA on day 42. Bronchoalveolar lavage fluid, lung tissues and serum samples were collected 6 days after the intranasal challenge.
Feeding of enriched milk fat led to marked suppression of airway inflammation as evidenced by reductions in eosinophilia and lymphocytosis in the airways, compared with feeding of normal milk fat and control diet. Enriched milk fat significantly reduced circulating allergen-specific IgE and IgG1 levels, together with reductions in bronchoalveolar lavage fluid of IL-5 and CCL11. Treatment significantly inhibited changes in the airway including airway epithelial cell hypertrophy, goblet cell metaplasia and mucus hypersecretion. The two major components of enriched milk fat, cis-9, trans-11 conjugated linoleic acid and VA, inhibited airway inflammation when fed together to mice, whereas alone they were not effective.
Milk fat enriched in conjugated linoleic and VAs suppresses inflammation and changes to the airways in an animal model of allergic airway disease.
有人认为，西方饮食中抗炎性不饱和脂质（如n-3多不饱和脂肪酸）的减少，导致了过敏性疾病的发病率和严重程度上升。
我们研究了给以牧草饲养的奶牛补充鱼和向日葵油所生产的富含共轭亚油酸和反式vaccenic酸（VAs）的乳脂肪（“富集”乳脂肪），是否能预防过敏性气道反应的发生。
给C57BL/6小鼠喂食含大豆油的对照饮食和补充了乳脂肪的饮食。在第14天和第28天通过腹腔注射卵清蛋白（OVA）使其致敏，并在第42天经鼻用OVA进行激发。鼻内激发后6天收集支气管肺泡灌洗液、肺组织和血清样本。
与喂食正常乳脂肪和对照饮食相比，喂食富集乳脂肪导致气道炎症明显受到抑制，气道中的嗜酸性粒细胞增多和淋巴细胞增多减少就是证明。富集乳脂肪显著降低了循环中过敏原特异性IgE和IgG1水平，同时支气管肺泡灌洗液中的IL-5和CCL11也减少。治疗显著抑制了气道的变化，包括气道上皮细胞肥大、杯状细胞化生和黏液分泌过多。富集乳脂肪的两个主要成分，顺式-9，反式-11共轭亚油酸和VAs，一起喂食小鼠时可抑制气道炎症，而单独使用时则无效。
富含共轭亚油酸和VAs的乳脂肪在过敏性气道疾病动物模型中可抑制炎症和气道变化。
