Fujimoto Yoshiko, Nomura Kenichi, Fukada Shuji, Shimizu Daisuke, Shimura Kazuho, Matsumoto Yosuke, Horiike Shigeo, Nishida Kazuhiro, Shimazaki Chihiro, Abe Masafumi, Taniwaki Masafumi
Department of Molecular Hematology and Oncology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan.
Eur J Haematol. 2008 Feb;80(2):143-50. doi: 10.1111/j.1600-0609.2007.00993.x. Epub 2008 Jan 1.
In non-Hodgkin's lymphoma (NHL), the majority of translocations involve the immunoglobulin heavy chain gene (IGH) locus, while a few involve the immunoglobulin light chain gene (IGL) locus, consisting of the kappa light chain gene (IGkappa) and the lambda light chain gene (IGlambda). Although many reports have dealt with the translocation and/or amplification of IGH in NHL, only a few have identified IGL translocations. To identify cytogenetic abnormalities and the partner chromosomes of IGL translocations in NHL, we performed dual-colour fluorescence in situ hybridisation (DC-FISH) and spectral karyotyping (SKY) in seven NHL cell lines and 40 patients with NHL. We detected IGL translocations in two cell lines and nine patients: four patients with diffuse large B-cell lymphoma, three with follicular lymphoma, one with extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue and one with mantle cell lymphoma. Five distinct partners of IGlambda translocation were identified by SKY analysis: 3q27 in three patients, and 1p13, 6p25, 17p11.2 and 17q21 in one patient each. Three cases featured double translocations of IGH and IGL. These findings warrant the identification of novel genes 1p13, 6p25, 17p11.2 and 17q21.
在非霍奇金淋巴瘤（NHL）中，大多数易位涉及免疫球蛋白重链基因（IGH）位点，少数涉及免疫球蛋白轻链基因（IGL）位点，后者由κ轻链基因（IGkappa）和λ轻链基因（IGlambda）组成。尽管许多报告涉及NHL中IGH的易位和/或扩增，但仅有少数报告鉴定出IGL易位。为了鉴定NHL中IGL易位的细胞遗传学异常及其伙伴染色体，我们对7株NHL细胞系和40例NHL患者进行了双色荧光原位杂交（DC-FISH）和光谱核型分析（SKY）。我们在2株细胞系和9例患者中检测到IGL易位：4例弥漫性大B细胞淋巴瘤患者、3例滤泡性淋巴瘤患者、1例黏膜相关淋巴组织结外边缘区B细胞淋巴瘤患者和1例套细胞淋巴瘤患者。通过SKY分析鉴定出5个不同的IGlambda易位伙伴：3例患者为3q27，其余4例患者分别为1p13、6p25、17p11.2和17q21。3例患者出现IGH和IGL的双重易位。这些发现为鉴定新基因1p13、6p25、17p11.2和17q21提供了依据。
