Silva Fabiana L, Rodrigues Antonio A M, Rego Isabela O P, Santos Raquel L H, Oliveira Raquel G, Silva Teane M A, Xavier Mariana N, Nascimento Ernane F, Santos Renato L
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, Brazil.
Vet Parasitol. 2008 Jan 25;151(1):86-90. doi: 10.1016/j.vetpar.2007.09.032. Epub 2007 Oct 7.
Recent reports indicate that Leishmania chagasi has tropism to the male canine genital system, which is associated with shedding of the organism in the semen, supporting the hypothesis of venereal transmission. The aim of this study was to describe the lesions and assess parasite load in the genital system of bitches with canine visceral leishmaniasis (CanL). Symptomatic (n=5) and asymptomatic (n=5) bitches seropositive for CanL were randomly selected at the Center for Zoonosis Control (Belo Horizonte, State of Minas Gerais, Brazil). Five serologically negative, healthy, adult bitches also from the CZC were used as controls. Samples from genital organs (vulva, vagina, cervix, uterine body, uterine horns, uterine tubes, and ovaries), liver, and spleen were histologically evaluated and processed for immunodetection of Leishmania sp., and PCR. The most significant histological change was a mild to moderate vulvar dermatitis, characterized by a histio-plasma-lymphocytic infiltrate. This change was detected in all asymptomatic, four symptomatic, and three uninfected control bitches. In one symptomatic and one asymptomatic bitch intracytoplasmic amastigotes were observed within macrophages in the inflammatory infiltrate. Samples from all the segments of the genital tract were positive in at least one infected animal, in the absence of detectable amastigotes in the tissue. These findings support the notion that L. chagasi does not have genital tropism in the bitch, which is in contrast to our previous findings in naturally infected male intact dogs.
近期报告表明，恰加斯利什曼原虫对雄性犬类生殖系统具有嗜性，这与该生物体在精液中的排出有关，支持了性传播的假说。本研究的目的是描述患有犬内脏利什曼病（CanL）的母犬生殖系统中的病变，并评估寄生虫负荷。在人畜共患病控制中心（巴西米纳斯吉拉斯州贝洛奥里藏特）随机选择了5只出现症状的和5只无症状的CanL血清学阳性母犬。还使用了同样来自该中心的5只血清学阴性、健康的成年母犬作为对照。对生殖器官（外阴、阴道、子宫颈、子宫体、子宫角、输卵管和卵巢）、肝脏和脾脏的样本进行组织学评估，并进行处理以免疫检测利什曼原虫属和进行聚合酶链反应（PCR）。最显著的组织学变化是轻度至中度的外阴皮炎，其特征为组织细胞 - 浆细胞 - 淋巴细胞浸润。在所有无症状母犬、4只出现症状的母犬和3只未感染的对照母犬中均检测到这种变化。在1只出现症状的母犬和1只无症状母犬的炎症浸润中的巨噬细胞内观察到胞浆内无鞭毛体。在至少一只感染动物中，生殖道所有节段的样本均呈阳性，而组织中未检测到无鞭毛体。这些发现支持了恰加斯利什曼原虫在母犬中不具有生殖嗜性的观点，这与我们之前在自然感染的雄性未阉割犬中的发现形成对比。
