Bruhn T O, Tresco P A, Jackson I M
Department of Medicine, Brown University/Rhode Island Hospital, Providence 02903.
Peptides. 1991 Sep-Oct;12(5):957-61. doi: 10.1016/0196-9781(91)90044-p.
Fetal (17-18 days of gestation) mediobasal hypothalamic tissue (MBH) was transplanted into the third ventricle of adult, male rats which had been treated neonatally with monosodium glutamate (MSG). MSG treatment caused a marked reduction of growth hormone-releasing factor-like-immunoreactive (GRF-i) perikarya in the arcuate nucleus and GRF-i fibers in the median eminence (ME), as compared to littermate controls. When normal fetal MBH was transplanted into the third ventricle of MSG recipients, numerous GRF-i perikarya were located within the graft four weeks following surgery. GRF-i fibers in the ME of MSG-treated rats were enhanced when MBH grafts were in close contact with the ME, but not when transplants were located dorsally or rostrally in the third ventricle without making contact with the recipient's ME. Fetal cerebral cortex, which was grafted as a control tissue, did not contain GRF-i neurons. These immunohistochemical results suggest that grafted fetal GRF-i perikarya may contact the recipient's ME to increase the content of GRF previously depleted by exposure to MSG.
将妊娠17 - 18天的胎儿中基底下丘脑组织（MBH）移植到成年雄性大鼠的第三脑室，这些大鼠在新生儿期用谷氨酸钠（MSG）处理过。与同窝对照相比，MSG处理导致弓状核中生长激素释放因子样免疫反应性（GRF-i）神经元胞体以及正中隆起（ME）中GRF-i纤维显著减少。当将正常胎儿MBH移植到MSG受体的第三脑室时，术后四周在移植物内发现大量GRF-i神经元胞体。当MBH移植物与ME紧密接触时，MSG处理大鼠ME中的GRF-i纤维增加，但当移植物位于第三脑室背侧或嘴侧且不与受体的ME接触时则没有增加。作为对照组织移植的胎儿大脑皮质不含GRF-i神经元。这些免疫组织化学结果表明，移植的胎儿GRF-i神经元胞体可能与受体的ME接触，以增加先前因接触MSG而耗尽的GRF含量。
