Merchenthaler I, Csontos C, Kallo I, Arimura A
Brain Res. 1986 Jul 23;378(2):297-302. doi: 10.1016/0006-8993(86)90932-7.
Two to 10 days after complete unilateral surgical isolation of the medial basal hypothalamus (MBH) or 3 months following neonatal monosodium glutamate (MSG) treatment, the presence of growth hormone-releasing hormone (GH-RH) immunoreactive neuronal structures was studied in rats using vibratome sections and GH-RH immunocytochemistry. Neonatal MSG treatment resulted in a dramatic decrease of GH-RH immunoreactivity in the median eminence (ME), but not complete disappearance as reported earlier. Unilateral complete deafferentation of the MBH caused only a slight decrease in GH-RH immunostaining in the posterior regions of the ipsilateral median eminence (ME). At this level GH-RH accumulation was observed in scattered transected fibers lateral to the cut, outside of the MBH. Our findings indicate that the arcuate nucleus is the major source of GH-RH immunoreactive structures in the ME. Although, however, in very small numbers, the existence of other sources of GH-RH terminals cannot be excluded.
在内侧基底下丘脑（MBH）完全单侧手术分离后的2至10天，或新生大鼠接受谷氨酸单钠（MSG）治疗3个月后，使用振动切片机切片和生长激素释放激素（GH-RH）免疫细胞化学方法，研究了大鼠中GH-RH免疫反应性神经元结构的存在情况。新生大鼠接受MSG治疗导致正中隆起（ME）中GH-RH免疫反应性显著降低，但并不像先前报道的那样完全消失。MBH单侧完全去传入神经仅导致同侧正中隆起（ME）后部区域的GH-RH免疫染色略有下降。在此水平上，在MBH外侧切断处外侧的散在横断纤维中观察到GH-RH积累。我们的研究结果表明，弓状核是ME中GH-RH免疫反应性结构的主要来源。然而，尽管数量极少，但不能排除GH-RH终末存在其他来源。
