Duke B O, Zea-Flores G, Muñoz B
Department of Infectious and Parasitic Disease Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000.
Trop Med Parasitol. 1991 Sep;42(3):175-80.
Adult Onchocerca volvulus, extracted from nodules before, and at intervals of two weeks to 12 months after, a single 150 micrograms/kg dose of ivermectin, were examined longitudinally and by sequential transverse sections. The mean number of male worms per nodule fell, and the proportion of nodules with no male worm rose, within two weeks of ivermectin and remained so for 12 months. In female worms, at intervals after ivermectin, the percentages of the length of the lower genital tracts occupied by embryos at each stage of development, or by degenerating ova, embryos and microfilariae (mfs), were recorded: (a) in un(re-)inseminated worms whose original embryogenesis was continuing and in those in which it was completed; and (b) in worms, reinseminated post-ivermectin, in which a new embryogenesis had begun. The results indicated that: (a) the time needed for the zygotes of O. volvulus to develop to mfs is 8-12 weeks; (b) nearly 40 percent of females had not resumed mf production by 12 months after treatment; (c) many intrauterine mfs had not degenerated within the first two weeks of ivermectin; (d) some of the last embryos to mature to mfs did not degenerate but accumulated temporarily in the anterior uteri 8-16 weeks after ivermectin.
从结节中提取的成年盘尾丝虫，在单次服用150微克/千克剂量的伊维菌素之前以及之后每隔两周至12个月进行纵向和连续横切面检查。在伊维菌素治疗后的两周内，每个结节中的雄虫平均数量下降，无雄虫的结节比例上升，并在12个月内保持这种状态。对于雌虫，在伊维菌素治疗后的不同时间间隔，记录下处于每个发育阶段的胚胎或退化的卵、胚胎和微丝蚴（mf）所占据的下生殖道长度的百分比：（a）在原始胚胎发生仍在继续的未（重新）受精雌虫以及胚胎发生已完成的雌虫中；（b）在伊维菌素治疗后重新受精且已开始新的胚胎发生的雌虫中。结果表明：（a）盘尾丝虫合子发育成微丝蚴所需的时间为8 - 12周；（b）近40%的雌虫在治疗后12个月时仍未恢复微丝蚴的产生；（c）许多子宫内的微丝蚴在伊维菌素治疗后的头两周内并未退化；（d）一些最后发育成熟为微丝蚴的胚胎并未退化，而是在伊维菌素治疗后8 - 16周暂时积聚在前子宫内。
