Noireau F, Lemesre J L, Vervoort T
Université Paris-Val de Marne, Créteil, France.
Trop Med Parasitol. 1991 Sep;42(3):195-6.
A total of 33 domestic animals living in close contact with man in a human trypanosomiasis focus in South Congo were examined parasitologically and tested for serological markers of Trypanosoma brucei gambiense infection. 84.8% of the animals presented detectable T. congolense parasitaemia. The high rate of seropositivity observed with CATT (81.8%) contrasted with the low seroprevalence found with ELISA (less than 13%). None of the 33 plasma samples showed lytic antibodies when analysed by immune lysis test against 10 distinct T. b. gambiense predominant variable antigen types (LiTat 1.1 to 1.10). The results demonstrate the lack of specificity of CATT, and to a lesser extent ELISA, in detecting T. b. gambiense infection in animals. The seropositivity may be due to cross-reaction with certain T. congolense antigens. The absence of serological markers specific to T. b. gambiense confirms the parasitological data which estimate the prevalence rate of animals infected with Trypanozoon as less than 1% in the region.
在刚果南部一个人类锥虫病疫源地，对与人类密切接触的33只家畜进行了寄生虫学检查，并检测了布氏冈比亚锥虫感染的血清学标志物。84.8%的动物呈现可检测到的刚果锥虫血症。用CATT检测观察到的高血清阳性率（81.8%）与用ELISA检测到的低血清流行率（低于13%）形成对比。在针对10种不同的布氏冈比亚锥虫主要可变抗原类型（LiTat 1.1至1.10）进行免疫裂解试验分析时，33份血浆样本中均未显示裂解抗体。结果表明，CATT在检测动物布氏冈比亚锥虫感染方面缺乏特异性，ELISA的特异性稍好一些。血清阳性可能是由于与某些刚果锥虫抗原发生交叉反应。缺乏布氏冈比亚锥虫特异性血清学标志物，这与寄生虫学数据一致，该数据估计该地区感染锥虫属的动物患病率低于1%。
