一种用于溶组织内阿米巴无菌培养的改良戴蒙德培养基。
A modification of Diamond's medium for the axenic culture of Entamoeba histolytica.
作者信息
Marinkelle C J, Guhl F, Aguirre A, Devia F
机构信息
Universidad de los Andes, Laboratorio de Microbiología y Parasitología, Bogotá, Colombia.
出版信息
Trans R Soc Trop Med Hyg. 1991 Nov-Dec;85(6):746-7. doi: 10.1016/0035-9203(91)90441-z.
PMID:1801343
Abstract
The use of casein hydrolysate in Diamond's axenic culture medium TPS-1 in replacement of trypticase allowed good growth of the trophozoites of Entamoeba histolytica. This modified medium also supported growth of trophozoites preserved for 16 months in liquid nitrogen. Considerable labour and cost of serum can be saved by using 5% instead of 10% bovine serum in combination with this modified medium.
摘要
在戴蒙德的无特定病原体培养基TPS - 1中使用酪蛋白水解物替代胰蛋白酶，可以使溶组织内阿米巴滋养体良好生长。这种改良培养基也支持在液氮中保存16个月的滋养体生长。通过在这种改良培养基中使用5%而非10%的牛血清，可以节省大量劳动力和血清成本。
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