Jin D K, Kohsaka T, Tanaka M, Abe J, Kobayashi N
Department of Immunology, National Children's Medical Research Center, Tokyo, Japan.
Acta Paediatr Jpn. 1991 Dec;33(6):709-13. doi: 10.1111/j.1442-200x.1991.tb02595.x.
An association of human leukocyte antigens (HLA) with idiopathic nephrotic syndrome (INS) in childhood was studied to characterize the immunogenetic background. We determined the class I antigens using the microlymphocytotoxicity test as well as class II antigens (DRB, DQB, DQA) and class III antigen (complement 4) using DNA restriction fragment length polymorphism (RFLP) in 36 children with INS. In the current study, neither the single allele in HLA class I antigen nor the single gene frequency in the class II region was significantly increased, in contrast to the results in Caucasian INS patients in whom a genetic predisposition to the HLA system, especially to HLA B8, DR3, and DR7, has been frequently reported. Also, in our data, the frequency of deletion of complement 4 gene was not different from that of the controls. These data suggest that there is a difference in immunogenetic background between Caucasian and Japanese INS patients as far as HLA is concerned.
为了明确免疫遗传背景，我们研究了人类白细胞抗原（HLA）与儿童特发性肾病综合征（INS）之间的关联。我们采用微量淋巴细胞毒性试验检测了36例INS患儿的Ⅰ类抗原，并运用DNA限制性片段长度多态性（RFLP）技术检测了Ⅱ类抗原（DRB、DQB、DQA）和Ⅲ类抗原（补体4）。在本研究中，与高加索INS患者的研究结果相反，HLAⅠ类抗原中的单个等位基因以及Ⅱ类区域中的单个基因频率均未显著增加，高加索INS患者中经常报道HLA系统存在遗传易感性，尤其是HLA B8、DR3和DR7。此外，在我们的数据中，补体4基因缺失的频率与对照组并无差异。这些数据表明，就HLA而言，高加索和日本INS患者的免疫遗传背景存在差异。
