[通过竞争性酶免疫测定法对细胞和组织匀浆中的人硫醇蛋白二硫键氧化还原酶进行免疫化学测定]
[Immunochemical determination of human thiol protein disulfide oxidoreductase in cell and tissue homogenates by competitive EIA].
作者信息
Kröning H, Mansfeld H W, Held C, Thiel U, Ansorge S
机构信息
Forschungsabteilung Experimentelle Immunologie, Medizinischen Akademie Magdeburg.
出版信息
Allerg Immunol (Leipz). 1991;37(2):89-96.
Different monospecific antisera against thiol-protein disulfide oxidoreductase (TPO, EC 1.8.4.2, protein-disulfide isomerase, EC 5.3.4.1) were raised in rabbits by immunization with purified human TPO and characterized by means of Laurell and immunoblot techniques. A competitive anti-TPO-EIA with insolubilized TPO has been used to determine this enzyme in cells and tissue homogenates. The assay shows a sensitivity of 1.2 ng/ml and a specificity of about 99%. The TPO content in relation to the total protein was found to be: in pancreas 0.65%, liver 0.45%, spleen 0.12%, placenta 0.16%, tonsils 0.06% and lymph nodes 0.03%.
通过用纯化的人硫醇 - 蛋白质二硫键氧化还原酶（TPO，EC 1.8.4.2，蛋白质二硫键异构酶，EC 5.3.4.1）免疫兔子，制备了不同的抗TPO单特异性抗血清，并通过劳雷尔（Laurell）法和免疫印迹技术进行了表征。一种使用固定化TPO的竞争性抗TPO - EIA已用于测定细胞和组织匀浆中的这种酶。该测定法的灵敏度为1.2 ng/ml，特异性约为99％。发现相对于总蛋白的TPO含量为：胰腺中0.65％，肝脏中0.45％，脾脏中0.12％，胎盘中0.16％，扁桃体中0.06％，淋巴结中0.03％。
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