Carmona M, Silva M, Pérez-Bendito D
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Analyst. 1991 Oct;116(10):1075-9. doi: 10.1039/an9911601075.
A stopped-flow method for the simultaneous determination of epinephrine and norepinephrine, two catecholamines with a wide spectrum of biological activity, is proposed. The method is based on the oxidation of these compounds with 1,10-phenanthroline-iron(III) complex, which is monitored by measuring the initial rate of change of the absorbance of the ferroin formed at 510 nm. The difference in kinetic behaviour between the two species was exploited by applying a modified version of the proportional-equation method for the resolution of epinephrine-norepinephrine mixtures at the micrograms ml-1 level over the ratio range 1:10-10:1, with an error of less than 5% and an average precision of about 2.5%. The method was successfully applied to the determination of the two catecholamines in pharmaceuticals.
提出了一种同时测定肾上腺素和去甲肾上腺素这两种具有广泛生物活性的儿茶酚胺的停流法。该方法基于这些化合物与1,10 - 菲啰啉 - 铁(III)络合物的氧化反应，通过测量在510 nm处形成的亚铁菲啰啉吸光度的初始变化速率来监测。利用这两种物质动力学行为的差异，应用比例方程法的改进版本，在微克每毫升水平上，对比例范围为1:10 - 10:1的肾上腺素 - 去甲肾上腺素混合物进行拆分，误差小于5%，平均精度约为2.5%。该方法成功应用于药物中两种儿茶酚胺的测定。
