Deftereos N T, Calokerinos A C, Efstathiou C E
Laboratory of Analytical Chemistry, University of Athens, Greece.
Analyst. 1993 Jun;118(6):627-32. doi: 10.1039/an9931800627.
A method is proposed for the determination of 0.0500-1.00 microgram ml-1 of epinephrine and L-dopa and 0.100-1.00 micrograms ml-1 of norepinephrine and dopamine by their chemiluminogenic oxidation with potassium permanganate in acidic medium, in the presence of formaldehyde, which greatly improves the sensitivity. Flow injection allows the measurement of 80 solutions per hour. The method was also optimized for a continuous-flow system. Comparative results from numerous organic compounds proved the necessity for electron-donating groups on the benzene ring for sensitive chemiluminescent characteristics.
提出了一种测定肾上腺素和L-多巴的方法，其浓度范围为0.0500 - 1.00微克/毫升，去甲肾上腺素和多巴胺的浓度范围为0.100 - 1.00微克/毫升。该方法是在酸性介质中，于甲醛存在的情况下，通过它们与高锰酸钾的化学发光氧化反应来进行测定的，甲醛的存在大大提高了灵敏度。流动注射法可实现每小时测定80个样品。该方法还针对连续流动系统进行了优化。众多有机化合物的对比结果证明，苯环上具有供电子基团对于灵敏的化学发光特性是必要的。
