Pauly J U, Heder A, Kurrle R, Seiler F R
Research Laboratories of Immunology, Behringwerke AG, Marburg/Lahn, Germany.
Behring Inst Mitt. 1991 Dec(90):104-11.
A sandwich enzyme immunoassay was developed for measuring human Interleukin 3 (IL-3) in human and animal sera. Polyclonal and monoclonal antibodies were raised against the recombinant human protein. These have been used to develop an immunoassay which can detect down to 10 pg/ml of human IL-3. The assay involves a polyclonal rabbit antibody coupled to a solid phase and a mouse monoclonal antibody-horseradish peroxidase conjugate as the detection antibody. Unlike the classical bone marrow assay and other cell line based bioassays for IL-3, the immunoassay was specific for the cytokine showing no or only negligible cross-reactivity with IL-1, IL-2, IL-6, erythropoietin, granulocyte colony-stimulating factor (G-CSF) and GM-CSF. The assay does not exhibit interfering matrix effects when used for the estimation of human IL-3 in serum samples.
开发了一种夹心酶免疫测定法，用于检测人和动物血清中的人白细胞介素3（IL-3）。制备了针对重组人蛋白的多克隆和单克隆抗体。这些抗体已用于开发一种免疫测定法，该方法可检测低至10 pg/ml的人IL-3。该测定法涉及与固相偶联的多克隆兔抗体和作为检测抗体的小鼠单克隆抗体-辣根过氧化物酶缀合物。与经典的骨髓测定法和其他基于细胞系的IL-3生物测定法不同，该免疫测定法对细胞因子具有特异性，与IL-1、IL-2、IL-6、促红细胞生成素、粒细胞集落刺激因子（G-CSF）和GM-CSF无交叉反应或交叉反应可忽略不计。当用于估计血清样品中的人IL-3时，该测定法不表现出干扰基质效应。
