Ishidoh K, Suzuki K, Katunuma N, Kominami E
Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan.
Biomed Biochim Acta. 1991;50(4-6):541-7.
The gene structures of rat cathepsins H and L have been determined. Cathepsin H gene spans more than 21.5 Kbp and comprises more than 12 exons. On the other hand, cathepsin L gene spans 8.5 Kbp and comprises 8 exons. In both genes, two intron insertion positions are conserved at the amino acid level. Both enzymes, therefore, diverged from a common ancestral gene. In the 5'-upstream region of the cathepsin L gene, no TATA box, one CAAT box and some SP-1 binding sites exist, common with other lysosomal enzyme genes. Furthermore, two AP-2 binding sites exist: a promoter under the control by the tumor promoter (TPA) and cAMP, and a cAMP response element (CRE). These results suggest that the cathepsin L gene expression is controlled by these factors.
大鼠组织蛋白酶H和L的基因结构已被确定。组织蛋白酶H基因跨度超过21.5千碱基对，包含12个以上的外显子。另一方面，组织蛋白酶L基因跨度为8.5千碱基对，包含8个外显子。在这两个基因中，两个内含子插入位置在氨基酸水平上是保守的。因此，这两种酶都源自一个共同的祖先基因。在组织蛋白酶L基因的5'-上游区域，不存在TATA盒，有一个CAAT盒和一些SP-1结合位点，这与其他溶酶体酶基因相同。此外，存在两个AP-2结合位点：一个受肿瘤启动子（TPA）和cAMP控制的启动子，以及一个cAMP反应元件（CRE）。这些结果表明，组织蛋白酶L基因的表达受这些因素的控制。
