Le Boulay C, Sellos D, Van Wormhoudt A
Station de Biologie Marine, Muséum National d'Histoire Naturelle et Collège de France, BP 225, 29910, Concarneau, France.
Gene. 1998 Sep 18;218(1-2):77-84. doi: 10.1016/s0378-1119(98)00385-0.
The gene structure of a cathepsin L of the shrimp Penaeus vannamei has been determined by the polymerase chain reaction. It comprises six exons of various lengths spanning a total of 1792bp. This architecture is homologous to that of rat cathepsin L, three conserved sites of intron position have been effectively identified, with the exception of the third intron break-point located immediately after the cysteinyl active site. In contrast, no similarity is observed with Drosophila or Plasmodium cathepsin L-like gene organizations. This gene expresses a major cathepsin L enzyme in the hepatopancreas. The last intron is polymorphic, suggesting the presence of at least three different genes.
凡纳滨对虾组织蛋白酶L的基因结构已通过聚合酶链反应确定。它由六个长度各异的外显子组成，总长1792bp。这种结构与大鼠组织蛋白酶L的结构同源，已有效鉴定出三个内含子位置的保守位点，但第三个内含子断点位于半胱氨酸活性位点之后。相比之下，未观察到与果蝇或疟原虫组织蛋白酶L样基因组织有相似性。该基因在肝胰腺中表达一种主要的组织蛋白酶L酶。最后一个内含子具有多态性，表明至少存在三个不同的基因。
