Kaplan A H, Swanstrom R
Department of Medicine, University of North Carolina, Chapel Hill 27599-7295.
Biomed Biochim Acta. 1991;50(4-6):647-53.
All retroviruses studied thus far contain proteases which process viral precursors to liberate the structural and enzymatic proteins of the viral capsid. We have examined the processing of the Gag precursor of HIV-1 which is composed of the viral structural proteins. Our results indicate that Gag is processed via two pathways: an expected membrane-associated pathway which gives rise to virions and a cytoplasmic pathway in which processed viral proteins accumulate in the cytoplasm. The presence of an active protease in the cytoplasm of infected cells is a potential source of toxicity. A comparison of the extent of cytoplasmic processing in lytically infected cells compared with that in cells which are not killed by the virus demonstrates a close correlation between cytoplasmic processing and cell killing.
迄今为止所研究的所有逆转录病毒都含有蛋白酶，这些蛋白酶加工病毒前体以释放病毒衣壳的结构蛋白和酶蛋白。我们已经研究了HIV-1的Gag前体的加工过程，该前体由病毒结构蛋白组成。我们的结果表明，Gag通过两条途径进行加工：一条是预期的与膜相关的途径，可产生病毒粒子；另一条是细胞质途径，加工后的病毒蛋白在细胞质中积累。感染细胞的细胞质中存在活性蛋白酶是潜在的毒性来源。与未被病毒杀死的细胞相比，对裂解感染细胞中细胞质加工程度的比较表明，细胞质加工与细胞死亡之间存在密切关联。
