Chang R S, Spina C A
J Infect Dis. 1976 Jun;133(6):676-80. doi: 10.1093/infdis/133.6.676.
Heat-inactivated preparations of Epstein-Barr virus stimulated human lymphocytes as assayed by incorporation of [(3)H]thymidine. The inactivated Epstein-Barr virus stimulated the lymphocytes of all five seropositive donors, 11 of 14 seronegative donors (aged eight to 26 years), and none of 15 neonates. Control antigens prepared from a human lymphoid cell line devoid of the Epstein-Barr virus genome did not stimulate the lymphocytes of seronegative donors. Fetal calf serum at the concentration used for suspension of Epstein-Barr virus did not stimulate or only minimally stimulated the lymphocytes of seronegative donors. The reactivity of the histocompatibility antigens found on human lymphocytes was abolished by procedures used for inactivation of the virus.
通过[（3）H]胸苷掺入法测定，爱泼斯坦-巴尔病毒的热灭活制剂可刺激人类淋巴细胞。灭活的爱泼斯坦-巴尔病毒刺激了所有5名血清阳性供体的淋巴细胞、14名血清阴性供体（年龄8至26岁）中的11名的淋巴细胞，而15名新生儿的淋巴细胞均未被刺激。从不含爱泼斯坦-巴尔病毒基因组的人类淋巴细胞系制备的对照抗原未刺激血清阴性供体的淋巴细胞。用于悬浮爱泼斯坦-巴尔病毒的浓度的胎牛血清未刺激或仅轻微刺激血清阴性供体的淋巴细胞。用于病毒灭活的程序消除了人类淋巴细胞上发现的组织相容性抗原的反应性。
