Weinman J, Della Gaspera B, Dautigny A, Pham Dinh D, Wang J, Nojima H, Weinman S
UFR Biomédicale des Saints-Pères Université René Descartes, Paris, France.
Cell Regul. 1991 Oct;2(10):819-26. doi: 10.1091/mbc.2.10.819.
Three different calmodulin genes that encode the identical protein have been identified in the rat (Nojima, 1989); however, calmodulin gene expression at the various stages of tissue differentiation and maturation has not been previously determined. We have quantitated the content of mRNAs encoding calmodulin in the developing brain and skeletal muscle using RNA blot analysis with three specific cDNA probes. Our results show that five species of calmodulin mRNAs: 4.0 and 1.7 kb for CaM I, 1.4 kb for CaM II, and 2.3 and 0.8 kb for CaM III are detectable at all ages in the brain as well as in skeletal muscle but exhibit a tissue-specific developmental pattern of expression. The comparison of the temporal pattern of calmodulin gene expression with both mitotic activity, as demonstrated by cyclin A mRNA levels, and differentiation and maturation of specific brain or muscle regions is consistent with calmodulin involvement in development.
在大鼠中已鉴定出三种编码相同蛋白质的不同钙调蛋白基因（野岛，1989年）；然而，以前尚未确定钙调蛋白基因在组织分化和成熟的各个阶段的表达情况。我们使用三种特异性cDNA探针通过RNA印迹分析对发育中的脑和骨骼肌中编码钙调蛋白的mRNA含量进行了定量。我们的结果表明，在脑和骨骼肌的所有年龄段都可检测到五种钙调蛋白mRNA：CaM I的4.0和1.7 kb、CaM II的1.4 kb以及CaM III的2.3和0.8 kb，但它们表现出组织特异性的发育表达模式。如细胞周期蛋白A mRNA水平所示，将钙调蛋白基因表达的时间模式与有丝分裂活性以及特定脑区或肌肉区域的分化和成熟进行比较，结果表明钙调蛋白参与了发育过程。
