Gandolfi F, Modina S, Brevini T A, Galli C, Moor R M, Lauria A
Istituto di Anatomia degli Animali Domestici, Università di Milano, Italy.
Eur J Basic Appl Histochem. 1991;35(4):383-92.
Previous studies have identified a glycoprotein (sOP 92) that is secreted by sheep oviductal epithelium and subsequently becomes associated with developing embryos. The objective of this study was to more definitively demonstrate the localization of this protein in the oviductal epithelium of cycling ewes and in the embryos (developmental stage ranging between 1 and 8 cells) by using the light and electron microscopic immuno-gold techniques. In the oviduct, sOP 92 was present only in nonciliated cells of the ampulla but not in the isthmus. No major differences in signal intensity, in its organ or intracellular distribution were observed between the postovulatory and the luteal phase of the cycle. At the embryonic level, sOP 92 uniformly covered the surface of the zona pellucida and permeated its width. Moreover, the protein was present in the perivitelline space and on the blastomeres membrane but not in the cytoplasm or in the nucleus. These observations suggest that a marked regional difference in sheep oviduct secretions exists and confirm that there is a close association of these secretions with the developing embryos.
以往的研究已鉴定出一种糖蛋白（sOP 92），它由绵羊输卵管上皮分泌，随后与发育中的胚胎相关联。本研究的目的是通过使用光镜和电镜免疫金技术，更确切地证明这种蛋白质在发情周期母羊输卵管上皮以及胚胎（发育阶段为1至8细胞）中的定位。在输卵管中，sOP 92仅存在于壶腹部的非纤毛细胞中，而峡部没有。在发情周期的排卵后期和黄体期之间，未观察到其信号强度、器官或细胞内分布的主要差异。在胚胎水平，sOP 92均匀覆盖透明带表面并渗透其宽度。此外，该蛋白质存在于卵周隙和卵裂球膜上，但不存在于细胞质或细胞核中。这些观察结果表明，绵羊输卵管分泌物存在明显的区域差异，并证实这些分泌物与发育中的胚胎密切相关。
