塞尔维亚严重少精子症不育男性Y染色体微缺失的患病率
Prevalence of Y chromosome microdeletions in infertile men with severe oligozoospermia in Serbia.
作者信息
Ristanovic M, Bunjevacki V, Tulic C, Novakovic I, Perovic V, Lukovic L J, Milasin J
机构信息
Institute of Human Genetics, Medical School, University of Belgrade, Belgrade, Serbia.
出版信息
Genet Couns. 2007;18(3):337-42.
AIM
The aim of this study was to determine the prevalence and type of microdeletions of the Y chromosome of men with severe oligozoospermia-ICSI candidates in the Serbian population and to compare our findings with those from other parts of the world.
METHODS
In all patients spermiogram has been performed in order to determine the sperm concentration. Patients were subjected to detailed clinical, endocrinological and cytogenetic examinations. Microdeletion analysis was performed by polymerase chain reaction (PCR) on 203 patients with normal cytogenetic findings. The STS markers tested in each case were sY84, sY86 (AZFa); sY127, sY134 (AZFb); sY254, sY255 (AZFc).
RESULTS
at least one of the STS markers was deleted in 11 of the 203 cases (5.4%).
CONCLUSION
AZFc microdeletions were identified with a rather high prevalence in men with severe oligozoospermia ICSI candidates in Serbian population.
目的
本研究旨在确定塞尔维亚人群中重度少精子症且适合进行卵胞浆内单精子注射（ICSI）的男性的Y染色体微缺失的患病率和类型，并将我们的研究结果与世界其他地区的结果进行比较。
方法
对所有患者进行精液分析以确定精子浓度。患者接受了详细的临床、内分泌和细胞遗传学检查。对203例细胞遗传学检查结果正常的患者采用聚合酶链反应（PCR）进行微缺失分析。每种情况下检测的序列标签位点（STS）标记为sY84、sY86（AZFa）；sY127、sY134（AZFb）；sY254、sY255（AZFc）。
结果
203例中有11例（5.4%）至少有一个STS标记缺失。
结论
在塞尔维亚人群中，重度少精子症且适合进行ICSI的男性中，AZFc微缺失的患病率相当高。
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