Zhao Xinfeng, Wang Yue, Sun Yuqing
Medical College, Dalian University, Dalian 116622, People's Republic of China.
J Chromatogr Sci. 2007 Sep;45(8):549-52. doi: 10.1093/chromsci/45.8.549.
A method is developed for the analysis of allantoin, gallic acid, dihydromelittoside, loganin, paeoniflorin, benzoylpaeoniflorin, and paeonol in Liuwei Dihuang tablets by high-performance liquid chromatography (HPLC)-UV-mass spectrometry (MS)-MS. Gradient elution with methanol-acetonitrile-water-formic acid solvent system is employed in the HPLC-electrospray ionization-MS study. The positive-ion ESI mode is suitable for these compounds. The peaks of gallic acid, loganin, dihydromelittoside, paeoniflorin, benzoylpaeoniflorin, and paeonol are identified by their mass spectra and the fragments of their MS-MS spectra. Allantoin, gallic acid, loganin, paeoniflorin, and paeonol are simultaneously determined by UV detection at 210 nm for quantitative purposes.
建立了一种采用高效液相色谱（HPLC）-紫外-质谱（MS）-质谱联用技术分析六味地黄丸中尿囊素、没食子酸、二氢蜜茱萸苷、马钱苷、芍药苷、苯甲酰芍药苷和丹皮酚的方法。在HPLC-电喷雾电离-质谱研究中采用甲醇-乙腈-水-甲酸溶剂系统进行梯度洗脱。正离子电喷雾电离模式适用于这些化合物。没食子酸、马钱苷、二氢蜜茱萸苷、芍药苷、苯甲酰芍药苷和丹皮酚的峰通过其质谱及其MS-MS谱的碎片进行鉴定。尿囊素、没食子酸、马钱苷、芍药苷和丹皮酚通过在210nm处的紫外检测同时进行定量测定。
