Diakowska Dorota, Lewandowski Andrzej, Kopeć Wacław, Diakowski Witold, Chrzanowska Teresa
Department of Gastrointestinal and General Surgery, Wrocław Medical University, Wrocław, Poland.
Hepatogastroenterology. 2007 Sep;54(78):1701-4.
BACKGROUND/AIMS: Oxidative stress is connected with activation of somatic mutations and rates of cell proliferation existing in cancer tissue. High level of reactive oxygen species is a mutagenic factor for DNA damage. Antioxidants are the components of the cellular defense mechanism against reactive oxygen molecules. The aim of our study was to analyze DNA peroxidation products' concentration and total antioxidant level in serum of the patients with esophageal squamous cell carcinoma before and after esophagectomy. We examined these parameters as markers of cancer development.
We tested 18 patients (2 woman and 16 men, mean age 59.4 years) with esophageal squamous cell cancer before and after esophagectomy and 12 healthy people as a control group. Concentrations of 8-OHdG and enzymatic antioxidants level were analyzed in serum. Data were statistically analyzed by Mann-Whitney test.
We observed statistically significant higher concentrations of 8-OHdG and significant lower levels of enzymatic antioxidants in the patients with cancer in comparison to the control group. After esophagectomy we observed normalization of these parameters. In four patients the level of total antioxidants was low and 8-OHdG concentration was high during the whole time of treatment. These patients had disease progression.
Estimation of serum 8-OHdG concentration and total antioxidant status may be helpful for monitoring cancer therapy in patients with esophageal squamous cell cancer.
背景/目的：氧化应激与癌症组织中存在的体细胞突变激活及细胞增殖速率相关。高水平的活性氧是导致DNA损伤的诱变因素。抗氧化剂是细胞对抗活性氧分子防御机制的组成部分。我们研究的目的是分析食管鳞状细胞癌患者在食管切除术前及术后血清中DNA过氧化产物的浓度和总抗氧化水平。我们将这些参数作为癌症发展的标志物进行检测。
我们对18例食管鳞状细胞癌患者（2例女性，16例男性，平均年龄59.4岁）在食管切除术前及术后进行了检测，并以12名健康人作为对照组。分析血清中8-羟基脱氧鸟苷（8-OHdG）的浓度和酶促抗氧化剂水平。数据采用曼-惠特尼检验进行统计学分析。
与对照组相比，我们观察到癌症患者血清中8-OHdG浓度在统计学上显著更高，而酶促抗氧化剂水平显著更低。食管切除术后，我们观察到这些参数恢复正常。在4例患者中，整个治疗期间总抗氧化剂水平较低，而8-OHdG浓度较高。这些患者出现了疾病进展。
评估血清8-OHdG浓度和总抗氧化状态可能有助于监测食管鳞状细胞癌患者的癌症治疗情况。
