Mota Javier, Chacon Juan C, Gutiérrez-Cabrera Ana E, Sánchez-Cordero Víctor, Wirtz Robert A, Ordoñez Rosalinda, Panzera Francisco, Ramsey Janine M
Centro de Investigaciones sobre Enfermedades Infecciosas (CISEI), Instituto Nacional de Salud Pública, Cuernavaca Morelos, Mexico.
Vector Borne Zoonotic Dis. 2007 Winter;7(4):617-27. doi: 10.1089/vbz.2007.0106.
Long-term control of triatomine bugs in Chagas endemic regions will depend on a full understanding of vector-parasite-host interactions. Herein we describe a cytochrome b multiplex polymerase chain reaction (PCR)-based strategy for blood meal source identification in bug foregut contents. This technique discriminates human from animal blood, and has been tested in five Triatoma species from México. Host identification has been validated for human, four rodent species, two bat species, dog, rabbit, sheep, and opossum. In addition, Trypanosoma cruzi can be identified simultaneously using S34/S67-specific kinetoplast DNA primers. Both host and parasite identification were possible as long as 10 weeks after bug feeding, and in samples stored up to 6 years. The blood meal identification procedure described here represents a powerful tool for large-scale studies identifying the biological, ecological, and environmental variables associated with Chagas disease transmission.
恰加斯病流行地区锥蝽的长期控制将取决于对媒介-寄生虫-宿主相互作用的全面理解。在此，我们描述了一种基于细胞色素b多重聚合酶链反应（PCR）的策略，用于鉴定锥蝽前肠内容物中的血餐来源。该技术可区分人类血液和动物血液，并已在来自墨西哥的五种锥蝽物种中进行了测试。宿主鉴定已在人类、四种啮齿动物物种、两种蝙蝠物种、狗、兔子、绵羊和负鼠中得到验证。此外，使用S34/S67特异性动基体DNA引物可同时鉴定克氏锥虫。在锥蝽进食后长达10周以及在储存长达6年的样本中，均可进行宿主和寄生虫鉴定。这里描述的血餐鉴定程序是大规模研究的有力工具，可用于识别与恰加斯病传播相关的生物学、生态学和环境变量。
