Rightor K S, Schmidt K L
Department of Pathology and Laboratory Medicine, University of Texas Medical School, Houston 77030.
Histol Histopathol. 1991 Apr;6(2):175-81.
Following ethanol exposure, the gastric surface epithelium often exfoliates, leaving a denuded basal lamina. Viable cells from the gland migrate along the basal lamina to repair the defect, a process known as restitution. Laminin, the major non-collagenous glycoprotein of basal laminae, functions in cellular adhesion and migration and, therefore, any alteration of this molecule by ethanol may influence subsequent restitution. After a 5 or 60 minutes treatment with saline, 50% or 100% ethanol, gastric tissues were removed from fasted female Sprague-Dawley rats, fixed in 1% paraformaldehyde and processed in Lowicryl. Once embedded and sectioned, specimens were incubated in anti-laminin followed by protein A-gold. The area of lamina densa from interfoveolar, pit and gland regions was measured and gold particles counted. Absolute ethanol caused diminished immunogold binding in all regions at all time periods, except the gland at 60 minutes. Exposure to 50% ethanol for 5 minutes did not alter laminin binding, although 60 minutes after 50% alcohol, laminin immunolabelling was increased. Alcohol concentration alters laminin immunogold binding, and therefore may influence restitution.
乙醇暴露后，胃表面上皮细胞常发生脱落，留下裸露的基膜。腺体内的活细胞沿基膜迁移以修复缺损，这一过程称为修复。层粘连蛋白是基膜的主要非胶原糖蛋白，在细胞黏附和迁移中起作用，因此乙醇对该分子的任何改变都可能影响随后的修复过程。用生理盐水、50%或100%乙醇处理5或60分钟后，从禁食的雌性斯普拉格-道利大鼠中取出胃组织，固定于1%多聚甲醛中，并用Lowicryl进行处理。包埋和切片后，标本先与抗层粘连蛋白抗体孵育，然后与蛋白A-金孵育。测量小窝间、小凹和腺体区域的致密层面积并计数金颗粒。无水乙醇在所有时间段均导致所有区域的免疫金结合减少，但60分钟时腺体区域除外。暴露于50%乙醇5分钟未改变层粘连蛋白结合，但在50%乙醇处理60分钟后，层粘连蛋白免疫标记增加。酒精浓度改变层粘连蛋白免疫金结合，因此可能影响修复过程。
