Lacy E R, Ito S
Lab Invest. 1984 Nov;51(5):573-83.
The superficial gastric epithelia of anesthetized rats were exposed to absolute ethanol for 30 to 45 seconds and fixed at intervals of 0, 3, 7, 15, 30, and 60 minutes thereafter. The repair or restitution process was evaluated by quantitative light microscopy and by transmission and scanning electron microscopy. The brief exposure to absolute ethanol destroyed more than 95% of the superficial epithelial cells without producing deeper, macroscopic, hemorrhagic lesions. Three minutes after ethanol treatment more than 90% of the superficial necrotic epithelial cells had broken loose from the mucosa and exposed the basal lamina. Within 7 minutes about 45% of the exposed basal lamina was recovered by migrating squamous-low cuboidal epithelial cells from the gastric pits. After 15 minutes about 85% of the damaged surface was already covered with mucous cells, and at 1 hour about 95% of the previously denuded basal lamina was recovered with more normal appearing columnar epithelial cells. Migrating mucous cells from the gastric pits extended large lamellipodia over the denuded basal lamina and moved at approximately 1 to 2 micron/minute. Exposure of the healing surface to 10(-4) M cytochalasin-D inhibited lamellipodial formation and impeded cell migration. In addition, this drug produced large lucent vacuoles in the persisting mucous cells. These results suggest that destruction of the normal superficial gastric mucosa of the rat may not be as detrimental as generally assumed since the repair process is extremely rapid and efficient.
将麻醉大鼠的胃表层上皮暴露于无水乙醇中30至45秒，然后分别在0、3、7、15、30和60分钟时进行固定。通过定量光学显微镜以及透射和扫描电子显微镜对修复或复原过程进行评估。短暂暴露于无水乙醇会破坏超过95%的表层上皮细胞，且不会产生更深的宏观出血性病变。乙醇处理3分钟后，超过90%的表层坏死上皮细胞从黏膜脱落，暴露出基膜。7分钟内，约45%暴露的基膜被来自胃小凹的鳞状 - 低立方上皮细胞迁移覆盖。15分钟后，约85%的受损表面已被黏液细胞覆盖，1小时时，约95%先前裸露的基膜被外观更正常的柱状上皮细胞覆盖。从胃小凹迁移的黏液细胞在裸露的基膜上伸出大的片状伪足，移动速度约为1至2微米/分钟。将愈合表面暴露于10(-4) M的细胞松弛素D会抑制片状伪足的形成并阻碍细胞迁移。此外，这种药物会在残留的黏液细胞中产生大的透明空泡。这些结果表明，大鼠正常胃表层黏膜的破坏可能不像通常认为的那样有害，因为修复过程极其迅速且高效。
