Dudek K, Kornasiewicz O, Koziak K, Kotulski M, Kalinowski P, Zieniewicz K, Krawczyk M
Department of General, Transplant and Liver Surgery, Medical University of Warsaw, Warsaw, Poland.
Transplant Proc. 2007 Nov;39(9):2788-92. doi: 10.1016/j.transproceed.2007.08.065.
Hepatocyte growth factor (HGF) plays a key role in the regulation of liver regeneration after hepatocyte damage. Changes in HGF gene expression reflect the status of the regeneration process.
The aim of this study was to ascertain the clinical significance of the expression of HGF among liver transplant patients.
Expression of the mRNA of HGF among peripheral blood lymphocytes were measured prior to as well as at 1, 2, 6, and 10 days after liver transplantation in a group of 30 liver recipients.
In first 24 hours after reperfusion, the patients with compromised graft function (group 1) showed persistently higher HGF gene expression after reperfusion compared with patients displaying well-functioning grafts (group 0; P = .0189). Between postoperative days 1 and 10, there was a rapid decrease in gene expression among group 0 compared with group 1 (P = .0155). The significant decrease observed in the both groups reached a certain plateau after 48 hours postoperatively. There was no statistical difference in aminotransaminase levels over the days after liver transplantation. The decreased mRNA HGF expression in lymphocytes preceded the decrease in aminotransferase levels.
HGF was more sensitive to predict early graft function than prothrombin time, aspartate aminotransferase, and alanine aminotransferase levels. The determination of HGF expression level in lymphocytes after liver transplantation may yield valuable information for evaluation of early graft function.
肝细胞生长因子（HGF）在肝细胞损伤后的肝再生调节中起关键作用。HGF基因表达的变化反映了再生过程的状态。
本研究的目的是确定肝移植患者中HGF表达的临床意义。
在一组30例肝移植受者中，于肝移植前以及移植后1、2、6和10天测量外周血淋巴细胞中HGF的mRNA表达。
在再灌注后的最初24小时内，移植功能受损的患者（第1组）与移植功能良好的患者（第0组）相比，再灌注后HGF基因表达持续更高（P = 0.0189）。在术后第1天至第10天之间，第0组的基因表达与第1组相比迅速下降（P = 0.0155）。两组中观察到的显著下降在术后48小时后达到一定平台期。肝移植后数天内转氨酶水平无统计学差异。淋巴细胞中mRNA HGF表达的下降先于转氨酶水平的下降。
HGF比凝血酶原时间、天冬氨酸转氨酶和丙氨酸转氨酶水平更能敏感地预测早期移植功能。肝移植后淋巴细胞中HGF表达水平的测定可能为评估早期移植功能提供有价值的信息。
