Kirby Alun C, Newton Darren J, Carding Simon R, Kaye Paul M
Immunology and Infection Unit, Hull York Medical School and Department of Biology, University of York, York, UK.
Eur J Immunol. 2007 Dec;37(12):3404-13. doi: 10.1002/eji.200737216.
Although gammadelta T cells are involved in the response to many pathogens, the dynamics and heterogeneity of the local gammadelta T cell response remains poorly defined. We recently identified gammadelta T cells as regulators of macrophages and dendritic cells during the resolution of Streptococcus pneumoniae-mediated lung inflammation. Here, using PCR, spectratype analysis and flow cytometry, we show that multiple gammadelta T cell subsets, including those bearing Vgamma1, Vgamma4 and Vgamma6 TCR, increase in number in the lungs of infected mice, but not in associated lymphoid tissue. These gammadelta T cells displayed signs of activation, as defined by CD69 and CD25 expression. In vivo BrdU incorporation suggested that local expansion, rather than recruitment, was the principal mechanism underlying this increase in gammadelta T cells. This conclusion was supported by the finding that pulmonary gammadelta T cells, but not alphabeta T cells, isolated from mice that had resolved infection exhibited lung-homing capacity in both naive and infected recipients. Together, these data provide novel insights into the origins of the heterogeneous gammadelta T cell response that accompanies lung infection, and the first evidence that inflammation-associated gammadelta T cells may exhibit distinct tissue-homing potential.
尽管γδ T细胞参与了对多种病原体的反应，但局部γδ T细胞反应的动态变化和异质性仍不清楚。我们最近发现，在肺炎链球菌介导的肺部炎症消退过程中，γδ T细胞是巨噬细胞和树突状细胞的调节因子。在此，我们利用聚合酶链反应（PCR）、谱型分析和流式细胞术表明，多个γδ T细胞亚群，包括那些携带Vγ1、Vγ4和Vγ6 TCR的亚群，在感染小鼠的肺部数量增加，但在相关淋巴组织中未增加。这些γδ T细胞表现出激活迹象，以CD69和CD25表达来定义。体内溴脱氧尿苷（BrdU）掺入表明，局部扩增而非募集是γδ T细胞数量增加的主要机制。从已解决感染的小鼠中分离出的肺部γδ T细胞而非αβ T细胞在未感染和感染的受体中均表现出肺归巢能力，这一发现支持了这一结论。总之，这些数据为伴随肺部感染的异质性γδ T细胞反应的起源提供了新的见解，并首次证明炎症相关的γδ T细胞可能表现出不同的组织归巢潜力。
