Chen Lee Chuin, Asakawa Daiki, Hori Hirokazu, Hiraoka Kenzo
Interdisciplinary Graduate School of Medical and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan.
Rapid Commun Mass Spectrom. 2007;21(24):4129-34. doi: 10.1002/rcm.3315.
Visible matrix-assisted laser desorption/ionization (VIS-MALDI) was performed using 2-amino-3-nitrophenol as matrix. The matrix is of near-neutral pH, and has an optical absorption band in the near-UV and visible region. A frequency-doubled Nd:YAG laser operated at 532 nm wavelength was used for matrix excitation and comparisons were made with a frequency-tripled Nd:YAG laser (355 nm). Visible and ultraviolet (UV)-MALDI produce similar mass spectra for peptides, polymers, and small proteins with comparable sensitivities. Due to the smaller optical absorption coefficient of the matrix at 532 nm wavelength, the optical penetration depth is larger, and the sample consumption per laser shot in VIS-MALDI is higher than that of UV-MALDI. Nevertheless, VIS-MALDI using 2-amino-3-nitrophenol as matrix may offer a complementary technique to the conventional UV-MALDI method in applications where deeper laser penetration is required.
使用2-氨基-3-硝基苯酚作为基质进行可见基质辅助激光解吸/电离（VIS-MALDI）。该基质接近中性pH值，并且在近紫外和可见光区域有一个光吸收带。使用波长为532 nm的倍频Nd:YAG激光进行基质激发，并与三倍频Nd:YAG激光（355 nm）进行比较。可见和紫外（UV）-MALDI对肽、聚合物和小蛋白质产生具有可比灵敏度的相似质谱。由于基质在532 nm波长处的光吸收系数较小，光穿透深度较大，VIS-MALDI中每次激光照射的样品消耗量高于UV-MALDI。然而，在需要更深激光穿透的应用中，使用2-氨基-3-硝基苯酚作为基质的VIS-MALDI可能为传统的UV-MALDI方法提供一种补充技术。
