Department of Chemistry, University of Western Ontario, London, Ontario, Canada.
J Am Soc Mass Spectrom. 2010 Feb;21(2):294-9. doi: 10.1016/j.jasms.2009.10.016. Epub 2009 Oct 29.
In this work, a new absorbing candidate, rhodamine (R) 575, is described, which forms the basis of a binary matrix operating at 532 nm. Analyte ionization is found to be much more efficient when the dye is combined with a proton donor such as hydrochloric acid or alpha-cyano-4-hydroxycinnamic acid, or a proton acceptor such as sodium hydroxide. This makes the matrix more generic than many others that have been tried. Furthermore, under visible illumination R575 produces very few chemical fragments, making it useful for small molecular weight analyte detection. Spectra for a variety of analytes are shown. Insight into the MALDI mechanism was obtained by comparing the similarities and differences of visible-MALDI with the more common UV and IR-MALDI strategies.
在这项工作中，描述了一种新的吸收候选物，若丹明（R）575，它构成了在 532nm 处工作的二元基质的基础。当染料与质子供体（如盐酸或α-氰基-4-羟基肉桂酸）或质子受体（如氢氧化钠）结合时，发现分析物的离子化效率要高得多。这使得该基质比许多已经尝试过的基质更通用。此外，在可见光照射下，R575 产生的化学碎片很少，因此可用于检测低分子量分析物。展示了各种分析物的光谱。通过比较可见光-MALDI 与更常见的 UV 和 IR-MALDI 策略之间的相似性和差异，获得了 MALDI 机制的深入了解。
