Zhang Zhongchun, Gao Xiang, Qiu Baosheng
College of Life Sciences, Central China Normal University, Wuhan, 430079 Hubei, PR China.
Phytochemistry. 2008 Feb;69(4):911-8. doi: 10.1016/j.phytochem.2007.10.012. Epub 2007 Nov 26.
Phytochelatins (PCs) are known to play an essential role in the heavy metal detoxification of some higher plants and fungi by chelating heavy metals. However, three recent papers reported that no PCs could be detected in the hyperaccumulator Sedum alfredii Hance upon cadmium, lead or zinc treatment, respectively. In this paper, PC synthesis was assayed again in the mine population of S. alfredii with the help of reversed phase high-performance liquid chromatography (HPLC), HPLC-mass spectrometry, and HPLC-tandem mass spectrometry. Our data showed that PC formation could be induced in the leaf, stem and root tissues of S. alfredii upon exposure to 400 microM cadmium, and only in the stem and root when exposed to 700 microM lead. However, no PCs were found in any part of S. alfredii when it was subjected to exposure to 1600 microM zinc.
已知植物螯合肽（PCs）通过螯合重金属在一些高等植物和真菌的重金属解毒过程中发挥重要作用。然而，最近有三篇论文分别报道，在镉、铅或锌处理后，超积累植物东南景天（Sedum alfredii Hance）中未检测到PCs。在本文中，借助反相高效液相色谱（HPLC）、HPLC-质谱联用以及HPLC-串联质谱联用技术，再次对东南景天矿区种群中的PC合成进行了测定。我们的数据表明，东南景天的叶、茎和根组织在暴露于400微摩尔镉时可诱导形成PCs，而在暴露于700微摩尔铅时仅在茎和根中诱导形成PCs。然而，当东南景天暴露于1600微摩尔锌时，在其任何部位均未发现PCs。
