用于测定镉胁迫条件下植物螯合肽和谷胱甘肽的高效液相色谱-蒸发光散射检测法的开发。
Development of the HPLC-ELSD method for the determination of phytochelatins and glutathione in under cadmium stress conditions.
作者信息
Zheng Xinyu, Chen Shen, Zheng Meiqin, Peng Jun, He Xiaosan, Han Yongming, Zhu Jingjing, Xiao Qingtie, Lv Rixin, Lin Ruiyu
机构信息
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China.
Key Laboratory of Crop Ecology and Molecular Physiology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China.
出版信息
R Soc Open Sci. 2018 May 9;5(5):171659. doi: 10.1098/rsos.171659. eCollection 2018 May.
A rapid, accurate and simple method was developed for the simultaneous determination of glutathione (GSH) and phytochelatins (PCs) by high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector. GSH, phytochelatin 2 (PC), PC, PC, PC and PC can be separated with baseline separation within 9 min using a Venusil AA column (250 mm × 4.6 mm i.d., 5 µm particle sizes). Acetonitrile and water containing 0.1% trifluoroacetic acid (0.1%) were employed as the mobile phase for the gradient elution. The drift tube temperature and flow rate of the carrier gas (N) were 50°C and 1.5 l min, respectively. Under optimum conditions, good linear regression equations of six analytes were obtained with the detection limits ranging from 0.2 to 0.5 µg ml. The proposed method has been applied successfully for the quantification of GSH and PCs in (a cadmium hyperaccumulator) under cadmium stress. The recoveries were between 82.9% and 115.3%.
建立了一种快速、准确且简便的方法，用于通过高效液相色谱（HPLC）结合蒸发光散射检测器同时测定谷胱甘肽（GSH）和植物螯合肽（PCs）。使用Venusil AA柱（250 mm×4.6 mm内径，5 µm粒径），可在9分钟内实现GSH、植物螯合肽2（PC2）、PC3、PC4、PC5和PC6的基线分离。以含有0.1%三氟乙酸（0.1%）的乙腈和水作为流动相进行梯度洗脱。漂移管温度和载气（N2）流速分别为50°C和1.5 l/min。在最佳条件下，六种分析物获得了良好的线性回归方程，检测限在0.2至0.5 µg/ml之间。所提出的方法已成功应用于镉胁迫下（一种镉超积累植物）中GSH和PCs的定量分析。回收率在82.9%至115.3%之间。
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