Li Pingzuo, Anumanthan Anukanth, Gao Xiu-Gong, Ilangovan Kuppusamy, Suzara Vincent V, Düzgüneş Nejat, Renugopalakrishnan V
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai, 200233, People's Republic of China.
Appl Biochem Biotechnol. 2007 Aug;142(2):105-24. doi: 10.1007/s12010-007-0003-x.
Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.
巴斯德毕赤酵母已被广泛且成功地用于表达重组蛋白。在本综述中，我们总结了表达异源蛋白所需的元件，并讨论了将该系统应用于蛋白表达的各种因素。这些元件包括载体、宿主菌株、异源基因整合到基因组中、分泌因子以及糖基化谱。特别是，我们讨论并评估了在优化发酵过程以提高表达蛋白的产量和稳定性方面的最新进展。可以通过控制培养基组成、pH值、温度和溶解氧，以及通过甲醇诱导和补料模式来实现优化。
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