Unver Yagmur, Ari Betul, Acar Melek, Yildiz Arslan Seyda
Department of Molecular Biology and Genetics, Faculty of Science, Atatürk University, Erzurum, Turkey.
Department of Molecular Biology and Genetics, Graduate School of Natural and Applied Sciences, Atatürk University, Erzurum, Turkey.
3 Biotech. 2024 Sep;14(9):193. doi: 10.1007/s13205-024-04039-x. Epub 2024 Aug 7.
(previously described as ) is a yeast that produces high-level heterologous proteins with a wide range of applications in medicine and industry. The methanol-induced alcohol oxidase I promoter (P) is frequently used for protein expression in this yeast. However, limitations on the use of methanol have been observed in large-scale production, including its flammability, toxicity, and need for special handling. Here, we propose to develop a system using recombinant cells constitutively expressing pectinmethyl esterase for expression of two reporter proteins, GFP and azurin, under the control of P using pectin in production medium. So, this system is coherent with yeast culture medium containing pectin and heterologous gene inserted downstream of P can be successfully expressed without the addition of methanol. Therefore, this novel Self-inducibLe heterologous protein EXpression (SILEX) system, which does not require the addition of methanol, can be used for the production of any protein. It can also be adapted for large-scale production.
The online version contains supplementary material available at 10.1007/s13205-024-04039-x.
（以前称为 ）是一种酵母，可产生高水平的异源蛋白，在医学和工业中有广泛应用。甲醇诱导的乙醇氧化酶I启动子（P）常用于该酵母中的蛋白质表达。然而，在大规模生产中已观察到甲醇使用的局限性，包括其易燃性、毒性以及需要特殊处理。在此，我们提议开发一种系统，该系统使用组成型表达果胶甲基酯酶的重组细胞，以便在生产培养基中使用果胶的情况下，在P的控制下表达两种报告蛋白，绿色荧光蛋白（GFP）和天青蛋白。因此，该系统与含有果胶的酵母培养基一致，并且插入P下游的异源基因无需添加甲醇即可成功表达。因此，这种无需添加甲醇的新型自诱导异源蛋白表达（SILEX）系统可用于生产任何蛋白质。它也可适用于大规模生产。
在线版本包含可在10.1007/s13205-024-04039-x获取的补充材料。
