A self-inducible heterologous protein expression system in ().

UNLABELLED

(previously described as ) is a yeast that produces high-level heterologous proteins with a wide range of applications in medicine and industry. The methanol-induced alcohol oxidase I promoter (P) is frequently used for protein expression in this yeast. However, limitations on the use of methanol have been observed in large-scale production, including its flammability, toxicity, and need for special handling. Here, we propose to develop a system using recombinant cells constitutively expressing pectinmethyl esterase for expression of two reporter proteins, GFP and azurin, under the control of P using pectin in production medium. So, this system is coherent with yeast culture medium containing pectin and heterologous gene inserted downstream of P can be successfully expressed without the addition of methanol. Therefore, this novel Self-inducibLe heterologous protein EXpression (SILEX) system, which does not require the addition of methanol, can be used for the production of any protein. It can also be adapted for large-scale production.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-024-04039-x.