Bunchu Nophawan, Sukontason Kabkaew L, Olson Jimmy K, Kurahashi Hiromu, Sukontason Kom
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Parasitol Res. 2008 Feb;102(3):419-29. doi: 10.1007/s00436-007-0780-8. Epub 2007 Nov 17.
Knowledge of the behavioral responses of medically important flies to natural stimuli is critical for the development of vector control strategies. We designed, constructed, and operated the dual-choice wind tunnel to investigate the behavioral responses of the blowfly, Chrysomya megacephala (Diptera: Calliphoridae), toward two important extrinsic factors (wind speed and olfactory stimuli) that affect the flight behavior of these flies. The dual-choice wind tunnel appeared as a "T-box," consisting of two stimulus partitions and one median release partition. The correlation between wind speed and fly response showed a marked relationship, with the wind speed set at 0.5 m/s being the optimal speed. Of the 72 natural products tested, 24--all having high protein content--attracted flies at the level of greater than 50% in the rearing cages during preliminary tests, thus allowing further investigation in the dual-choice wind tunnel to further assess these responses. The behavioral response between flies exposed to the screening odors in a rearing cage and those exposed in the dual-choice wind tunnel showed a similar trend. One-day-tainted pork viscera yielded the greatest positive response among natural products tested, attracting greater than 90% of the flies included in the test population. One-day-tainted pork viscera exhibited the highest potency (1.578), exceeding 1-day-tainted beef liver, which is commonly used as the standard bait for blowflies. The usefulness and practical applications of the dual-choice wind tunnel for further investigations are also discussed.
了解具有医学重要性的苍蝇对自然刺激的行为反应对于制定病媒控制策略至关重要。我们设计、建造并操作了双选风洞，以研究大头金蝇（Chrysomya megacephala，双翅目：丽蝇科）对影响这些苍蝇飞行行为的两个重要外在因素（风速和嗅觉刺激）的行为反应。双选风洞呈现为一个“T型箱”，由两个刺激分区和一个中间释放分区组成。风速与苍蝇反应之间的相关性显示出显著关系，设定为0.5米/秒的风速是最佳速度。在测试的72种天然产物中，有24种——均具有高蛋白含量——在初步测试期间在饲养笼中吸引苍蝇的比例超过50%，因此可在双选风洞中进一步研究以进一步评估这些反应。在饲养笼中暴露于筛选气味的苍蝇与在双选风洞中暴露的苍蝇之间的行为反应呈现出相似趋势。在测试的天然产物中，一日变质的猪内脏产生的阳性反应最大，吸引了测试群体中超过90%的苍蝇。一日变质的猪内脏表现出最高的效力（1.578），超过了通常用作大头金蝇标准诱饵的一日变质牛肝。还讨论了双选风洞在进一步研究中的实用性和实际应用。
