Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Parasitol Res. 2013 Nov;112(11):3789-98. doi: 10.1007/s00436-013-3566-1. Epub 2013 Aug 27.
The behavioral response of flies to olfactory cues remains the focus of many investigations, and wind tunnels have sometimes been employed for assessment of this variable in the laboratory. In this study, our aim was to design, construct, and operate a new model of I-box wind tunnel with improved efficacy, highlighting the use of a new wind tunnel model to investigate the behavioral response of the medically important blow fly, Chrysomya megacephala (Fabricius). The I-box dual-choice wind tunnel designed for this study consists of seven conjoined compartments that resulted in a linear apparatus with clear glass tunnel of 30 × 30 × 190 cm ended both sides with wooden "fan compartments" which are equipped with adjustable fans as wind source. The clear glass tunnel consisted of two "stimulus compartments" with either presence or absence (control) of bait; two "trap compartments" where flies were attracted and allowed to reside; and one central "release compartment" where flies were introduced. Wind tunnel experiments were carried out in a temperature-controlled room, with a room light as a light source and a room-ventilated fan as odor-remover from tunnel out. Evaluation of testing parameters revealed that the highest attractive index was achieved with the use of 300 g of 1-day tainted pork scrap (pork meat mixed with offal) as bait in wind tunnel settings wind speed of 0.58 m/s, during 1.00-5.00 PM with light intensity of 341.33 lux from vertical light and 135.93 lux from horizontal light for testing a group of 60 flies. In addition, no significant response of well-fed and 24 h staved flies to this bait under these conditions was found. Results of this study supported this new wind tunnel model as a suitable apparatus for investigation of behavioral response of blow flies to bait chemical cues in the laboratory.
蝇类对嗅觉线索的行为反应仍然是许多研究的重点，有时在实验室中使用风洞来评估这个变量。在这项研究中，我们的目的是设计、构建和操作一种新的 I 型箱风洞模型，以提高其效果，强调使用新型风洞模型来研究具有医学重要性的丽蝇 Chrysomya megacephala (Fabricius) 的行为反应。为这项研究设计的 I 型箱双选择风洞由七个连接的隔室组成，形成了一个线性装置，带有 30×30×190cm 的透明玻璃隧道，两端都有木制的“风扇隔室”，里面装有可调节的风扇作为风源。透明玻璃隧道由两个“刺激隔室”组成，一个隔室有诱饵，另一个隔室没有（对照）；两个“陷阱隔室”，蝇类被吸引并允许居住；一个中央“释放隔室”，蝇类被引入。风洞实验在温度可控的房间中进行，房间灯光作为光源，房间通风扇作为从隧道中去除气味的装置。测试参数的评估表明，在风速为 0.58m/s、下午 1.00-5.00 点、垂直光照强度为 341.33 勒克斯、水平光照强度为 135.93 勒克斯的风洞设置下，使用 300 克 1 天污染的猪肉屑（猪肉与内脏混合）作为诱饵时，获得了最高的吸引力指数。此外，在这些条件下，吃饱的和 24 小时禁食的蝇类对这种诱饵没有明显的反应。这项研究的结果支持这种新型风洞模型作为一种适合在实验室中研究丽蝇对诱饵化学线索的行为反应的设备。
