供体血浆中基质金属蛋白酶-2的水平与储存血小板浓缩物的输血后恢复密切相关。
The level of matrix metalloproteinase-2 in donor plasma strongly correlates with post-transfusion recovery of stored platelet concentrates.
作者信息
Mutlu A, Allen D J, Freedman J, Leytin V
机构信息
Division of Transfusion Medicine, Department of Laboratory Medicine, The Keenan Research Centre in the Li Ka Shing Knowledge Institute of St Michael's Hospital, Toronto, ON, Canada.
出版信息
Vox Sang. 2008 Feb;94(2):143-5. doi: 10.1111/j.1423-0410.2007.01001.x. Epub 2007 Nov 19.
BACKGROUND AND OBJECTIVE
A simple test accurately predicting post-transfusion recovery of stored platelet concentrates (PCs) is needed.
MATERIALS AND METHODS
Matrix metalloproteinase-2 (MMP-2) was determined in fresh-frozen plasma of normal donors using enzyme-linked immunosorbent assay (ELISA)-based assay. Post-transfusion recovery of human PCs was determined in rabbits with an inhibited reticuloendothelial system by flow cytometry.
RESULTS
We demonstrated a strong positive (r = 0.91) and highly significant (P = 0.0015) correlation between the concentration of MMP-2 in plasma of normal donors and post-transfusion recovery of PCs stored for 5 days at 22 degrees C under standard blood banking conditions. A higher level of donor plasma MMP-2 is associated with improved recovery of transfused platelets.
CONCLUSION
These observations suggest that pre-transfusion MMP-2 assay of donor's plasma may be a predictor of post-transfusion recovery of PCs. However, the usefulness of this assay should be elucidated in clinical transfusion settings.
背景与目的
需要一种能准确预测储存血小板浓缩物（PCs）输血后恢复情况的简单检测方法。
材料与方法
使用基于酶联免疫吸附测定（ELISA）的方法，测定正常献血者新鲜冰冻血浆中的基质金属蛋白酶-2（MMP-2）。通过流式细胞术，在网状内皮系统受抑制的兔体内测定人PCs的输血后恢复情况。
结果
我们证明，在标准血库条件下于22℃储存5天的PCs，正常献血者血浆中MMP-2浓度与输血后恢复情况之间存在强正相关（r = 0.91）且高度显著相关（P = 0.0015）。供体血浆中MMP-2水平较高与输注血小板的恢复情况改善相关。
结论
这些观察结果表明，输血前检测供体血浆中的MMP-2可能是PCs输血后恢复情况的一个预测指标。然而，该检测方法在临床输血环境中的实用性尚需阐明。
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